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b7 h3 targeted car  (ATCC)


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    ATCC b7 h3 targeted car
    B7 H3 Targeted Car, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b7+h3/B-7/10__1158_slash_1078___0432__ccr___26___0738-67-9-35
    Average 96 stars, based on 527 article reviews
    b7 h3 targeted car - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Produced:

    Article Title: B7-H3–Targeting Chimeric Antigen Receptors Epstein-Barr Virus–specific T Cells Provides a Tumor Agnostic Off-The-Shelf Therapy Against B7-H3–positive Solid Tumors
    Article Snippet: On the basis of published work ( ) indicating superior fitness in murine T cell–expressing CARs with reduced number of Immunoreceptor Tyrosine-based Activation Motif (ITAM) domains, we also inactivated the second and third ITAMs of the murine CD3ζ molecule by site-directed mutagenesis (Takara Bio). .. Retrovirus vectors carrying the B7-H3–targeting human and murine CARs were produced in RD114 (BioVec Pharma) or Phoenix Eco (ATCC) packaging cell lines respectively, by transient transfection using PEIpro transfection reagent (Polyplus). .. Media containing retroviral vectors were harvested 48 and 72 hours posttransfection and concentrated using RetroX Concentrator (Takara Bio) before snap frozen and stored at −80°C.

    Article Title: B7-H3-Targeting Chimeric Antigen Receptors Epstein Barr Virus Specific T Cells Provides a Tumor Agnostic Off-The-Shelf Therapy Against B7-H3 Positive Solid Tumors
    Article Snippet: On the basis of published work (22) indicating superior fitness in murine T cell–expressing CARs with reduced number of Immunoreceptor Tyrosine-based Activation Motif (ITAM) domains, we also inactivated the second and third ITAMs of the murine CD3ζ molecule by site-directed mutagenesis (Takara Bio). .. Retrovirus vectors carrying the B7-H3–targeting human and murine CARs were produced in RD114 (BioVec Pharma) or Phoenix Eco (ATCC) packaging cell lines respectively, by transient transfection using PEIpro transfection reagent (Polyplus). .. Media containing retroviral vectors were harvested 48 and 72 hours posttransfection and concentrated usingRetroXConcentrator (Takara Bio) before snap frozen and stored at −80°C.

    Transfection:

    Article Title: B7-H3–Targeting Chimeric Antigen Receptors Epstein-Barr Virus–specific T Cells Provides a Tumor Agnostic Off-The-Shelf Therapy Against B7-H3–positive Solid Tumors
    Article Snippet: On the basis of published work ( ) indicating superior fitness in murine T cell–expressing CARs with reduced number of Immunoreceptor Tyrosine-based Activation Motif (ITAM) domains, we also inactivated the second and third ITAMs of the murine CD3ζ molecule by site-directed mutagenesis (Takara Bio). .. Retrovirus vectors carrying the B7-H3–targeting human and murine CARs were produced in RD114 (BioVec Pharma) or Phoenix Eco (ATCC) packaging cell lines respectively, by transient transfection using PEIpro transfection reagent (Polyplus). .. Media containing retroviral vectors were harvested 48 and 72 hours posttransfection and concentrated using RetroX Concentrator (Takara Bio) before snap frozen and stored at −80°C.

    Article Title: B7-H3-Targeting Chimeric Antigen Receptors Epstein Barr Virus Specific T Cells Provides a Tumor Agnostic Off-The-Shelf Therapy Against B7-H3 Positive Solid Tumors
    Article Snippet: On the basis of published work (22) indicating superior fitness in murine T cell–expressing CARs with reduced number of Immunoreceptor Tyrosine-based Activation Motif (ITAM) domains, we also inactivated the second and third ITAMs of the murine CD3ζ molecule by site-directed mutagenesis (Takara Bio). .. Retrovirus vectors carrying the B7-H3–targeting human and murine CARs were produced in RD114 (BioVec Pharma) or Phoenix Eco (ATCC) packaging cell lines respectively, by transient transfection using PEIpro transfection reagent (Polyplus). .. Media containing retroviral vectors were harvested 48 and 72 hours posttransfection and concentrated usingRetroXConcentrator (Takara Bio) before snap frozen and stored at −80°C.

    In Vitro:

    Article Title: Comparison of approaches for increasing affinity of affibody molecules for imaging of B7-H3: dimerization and affinity maturation
    Article Snippet: A dose calibrator VDC-405 (Veenstra Instruments BV, Joure, The Netherlands) equipped with an ionization chamber was utilised for measuring the radioactivity for labelling and injection formulation. .. B7-H3-expressing ovarian cancer SKOV-3 and breast cancer BT-474 cell lines, obtained from the American Type Culture Collection (ATCC), was used for in vitro cell studies. .. Ramos lymphoma cells (ATCC) were used to establish B7-H3-negative xenografts.

    Article Title: Comparison of approaches for increasing affinity of affibody molecules for imaging of B7-H3: dimerization and affinity maturation.
    Article Snippet: A dose calibrator VDC-405 (Veenstra Instruments BV, Joure, The Netherlands) equipped with an ionization chamber was utilised for measuring the radioactivity for labelling and injection formulation. .. B7-H3-expressing ovarian cancer SKOV-3 and breast cancer BT-474 cell lines, obtained from the American Type Culture Collection (ATCC), was used for in vitro cell studies. .. Ramos lymphoma cells (ATCC) were used to establish B7-H3-negative xenografts.

    Article Title: Efficient 68 Ga Labeling of a B7-H3-Targeting Affibody Molecule via Acyclic Tris(hydroxypyridinone) Chelator: Effects on Biodistribution in a Preclinical Model
    Article Snippet: .. In vitro cell studies were performed using the B7-H3-expressing ovarian cancer SKOV-3 and breast cancer BT-474 cell lines, obtained from the American Type Culture Collection (ATCC). ..

    Article Title: Efficient 68 Ga Labeling of a B7-H3-Targeting Affibody Molecule via Acyclic Tris(hydroxypyridinone) Chelator: Effects on Biodistribution in a Preclinical Model.
    Article Snippet: .. In vitro cell studies were performed using the B7-H3-expressing ovarian cancer SKOV-3 and breast cancer BT-474 cell lines, obtained from the American Type Culture Collection (ATCC). ..



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    Image Search Results


    SIRT1 acts as a downstream regulator of CD276 in mediating E-Cad. (A) The expression of SIRT1 was analyzed by western blotting in HepLi5, SNU-449 and 3B cell lines under sparse or confluent conditions. (B) The expression of SIRT1 in 449-OX cells was analyzed by western blotting under three conditions: sparse, confluent, and confluent with additional treatment of LY294002. (C) The expression of SIRT1 was analyzed by western blotting in 3B-KD cells under sparse and confluent conditions. 3B, Hep 3B2.1-7; 449-OX, CD276-ovexpressing SNU-449 cells; Li5, HepLi5; 449, SNU-449; 3B, Hep 3B2.1-7; S, sparse cultures; C, confluent cultures; LY, LY294002; si-NC, small interfering-negative control; 3B-KD, CD276-knockdown Hep 3B2.1-7 cells.

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    Article Title: A cascade via CD276/PI3K/SIRT1/E-Cad in overcoming contact inhibition of proliferation in hepatocellular carcinoma cells

    doi: 10.3892/mco.2026.2941

    Figure Lengend Snippet: SIRT1 acts as a downstream regulator of CD276 in mediating E-Cad. (A) The expression of SIRT1 was analyzed by western blotting in HepLi5, SNU-449 and 3B cell lines under sparse or confluent conditions. (B) The expression of SIRT1 in 449-OX cells was analyzed by western blotting under three conditions: sparse, confluent, and confluent with additional treatment of LY294002. (C) The expression of SIRT1 was analyzed by western blotting in 3B-KD cells under sparse and confluent conditions. 3B, Hep 3B2.1-7; 449-OX, CD276-ovexpressing SNU-449 cells; Li5, HepLi5; 449, SNU-449; 3B, Hep 3B2.1-7; S, sparse cultures; C, confluent cultures; LY, LY294002; si-NC, small interfering-negative control; 3B-KD, CD276-knockdown Hep 3B2.1-7 cells.

    Article Snippet: Membranes were then blocked with 5% non-fat milk for 1 h at room temperature and incubated overnight at 4 ̊C with primary antibodies: CD276 (1:1,000; cat. no. A17216), E-Cad (1:2,000; cat. no. A24874), p27 (1:1,000; cat. no. A0290), cyclin D1 (1:1,000; cat. no. A11310), GAPDH (1:50,000; cat. no. A19056) and SIRT1 (1:2,000; cat. no. A0230; all from ABclonal Biotech Co., Ltd.).

    Techniques: Expressing, Western Blot, Negative Control, Knockdown

    A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.

    Journal: bioRxiv

    Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors

    doi: 10.64898/2026.04.15.718753

    Figure Lengend Snippet: A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.

    Article Snippet: Primary human anti–B7-H3 antibody (AF1027, R&D Systems) was diluted in cold staining buffer (0.5 μg/μL; 2 μL per 100 μL reaction volume) and applied to cells for 20–30 min at 4 °C.

    Techniques: Immunocytochemistry, Expressing, Fluorescence, Co-Culture Assay

    Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.

    Journal: bioRxiv

    Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors

    doi: 10.64898/2026.04.15.718753

    Figure Lengend Snippet: Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.

    Article Snippet: Primary human anti–B7-H3 antibody (AF1027, R&D Systems) was diluted in cold staining buffer (0.5 μg/μL; 2 μL per 100 μL reaction volume) and applied to cells for 20–30 min at 4 °C.

    Techniques: Activity Assay, Imaging